A. Drug effects on auditory disorders
1. Sensorineural hearing loss
2. Tinnitus

B. Drug effects on metabolic diseases

C. Zebrafish disease models

1: Pham TNM, Jeong SY, Kim DH, Park YH, Lee JS, Lee KW, Moon IS, Choung SY, Kim SH, Kang TH, Jeong KW. Protective Mechanisms of Avocado Oil Extract Against Ototoxicity. Nutrients. 2020 Mar 29;12(4):947. doi: 10.3390/nu12040947. PMID: 32235401; PMCID: PMC7230542.

2: Castañeda R, Natarajan S, Jeong SY, Hong BN, Kang TH. Traditional oriental medicine for sensorineural hearing loss: Can ethnopharmacology contribute to potential drug discovery? J Ethnopharmacol. 2019 Mar 1;231:409-428. doi: 10.1016/j.jep.2018.11.016. Epub 2018 Nov 12. PMID: 30439402.

3: Ji MG, Lee YR, Nam YH, Castañeda R, Hong BN, Kang TH. Immunostimulatory Action of High-Content Active Arabinoxylan in Rice Bran. ACS Omega. 2020 Sep22;5(41):26374-26381. doi: 10.1021/acsomega.0c02472. PMID: 33110965; PMCID: PMC7581081.

4: Castañeda R, Natarajan S, Jeong SY, Hong BN, Kang TH. Electrophysiological changes in auditory evoked potentials in rats with salicylate-induced tinnitus. Brain Res. 2019 Jul 15;1715:235-244. doi: 10.1016/j.brainres.2019.04.004. Epub 2019 Apr 5. PMID: 30958992.

5: Kim DH, Park YH, Lee JS, Jeong HI, Lee KW, Kang TH. Anti-Obesity Effect of DKB-117 through the Inhibition of Pancreatic Lipase and α-Amylase Activity. Nutrients. 2020 Oct 6;12(10):3053. doi: 10.3390/nu12103053. PMID: 33036193; PMCID: PMC7600053.

6: Shin JK, Park JH, Kim KS, Kang TH, Kim HS. Antiulcer Activity of Steamed Ginger Extract against Ethanol/HCl-Induced Gastric Mucosal Injury in Rats.Molecules. 2020 Oct 13;25(20):4663. doi: 10.3390/molecules25204663. PMID: 33066164; PMCID: PMC7587366.

1. Neurodevelopmental toxicity
2. Undiagnosed rare diseases
3. In vivo animal models for targeted protein degradation

Jung SH, Kim J, Eum J, Choe JW, Kim HH, Kee Y*, and Lee K* (2019) Velutin, an Aglycone Extracted from Korean Mistletoe, with Improved Inhibitory Activity against Melanin Biosynthesis. Molecules, 24, pii E2549
Ki S, Kwon S-H, Eum J, Raslan AA, Kim K-N, Hwang BJ*, and Kee Y* (2019) 3D light-sheet assay assessing novel valproate-associated cardiotoxicity and folic acid relief in zebrafish embryogenesis. Chemosphere, 227, 551-560
Park SK, Hwang BJ, and Kee Y* (2019) Promoter cross-talk affects the inducible expression of intronic shRNAs from the tetracycline response element. Genes Genomics, 41, 483-490
Park SK, Kee Y, and Hwang BJ (2019) Enhancement of gene knockdown efficiency by CNNC motifs in the intronic shRNA precursor. Genes Genomics, 41, 491-498
Heo H, Jo J, Jung JI, Han YM, Lee S, Kim SR, Kwon SH, Kim KN, Hwang BJ, Kee Y, Lee BD, Kang D, and Her S (2019) Improved dynamic monitoring of transcriptional activity during longitudinal analysis in the mouse brain. Biol Open, Jan 8;8(1). pii: bio037168. doi: 10.1242/bio.037168
Park S.K., Kee Y., Ryu T., Kim H, and Hwang BJ (2019) Enzymatic construction of shRNA library from oligonucleotide library. Genes Genomics, doi: 10.1007/s13258-019-00800-2
Hwang BJ and Kee Y (2016) Small molecule inhibition of speckle-type POZ protein-substrate interactions for the treatment of renal cell carcinoma. Transl Cancer Res. 5 (Suppl 7): S1509-S1514
Eum J, Kwak J, Kim HJ, Ki S, Lee K, Raslan AA, Park OK, Chowdhury MAU, Her S, Kee Y, Kwon S, and Hwang BJ (2016) 3D visualization of developmental toxicity of 2,4,6-trinitrotoluene in zebrafish embryogenesis using light-sheet microscopy. Int J Mol Sci, 17, 1925(1-14)
Park OK, Kwak J, Jung YJ, Kim YH, Hong H, Hwang BJ, Kwon S*, and Kee Y* (2015) 3D light-sheet fluorescence microscopy of cranial neurons and vasculature during zebrafish embryogenesis. Mol Cells, 38(11), 975-981
Shin, Y.J., Park, S.K., Jung, Y.J., Kim, Y.N., Kim, K.S., Park, O.K., Kwon, S., Jeon, S.H., Trinh, L.A., Fraser, S.E., Kee, Y.*, and Hwang, B.j.* (2015) Nanobody-targeted E3-ubiquitin ligase complex degrades nuclear proteins. Scientific Reports, 5:14269
Kim, M., Cho, H., Kwon, G., Kang, Y., Kwon, S., Her, S., Kee, Y., Park, J.H.Y. (2015) Benzyl isothiocyanate suppresses high-fat diet-stimulated mammary tumor progression via the alteration of tumor microenvironments in obesity-resistant BALB/c mice. Mol Carcinogen, 54(1), 72-82
Kwon, S., Park, O.K., Nie, S., Kwak, J., Hwang, B.J., Bronner, M.E. and Kee, Y.* (2014) Bioinformatic analysis of nematode migration-associated genes identifies novel vertebrate neural crest markers. PLOS One, 9(7), e103024
Islam A.M.T., Kwak, J., Jung, Y.J., and Kee, Y.* (2014) Animal models of Amyotrophic lateral sclerosis and Huntington’s disease. Genes Genomics 36, 399-413
Raslan, A.A. and Kee, Y.* (2013) Tackling neurodegenerative disease: animal models of Alzheimer’s disease and Parkinson’s disease. Genes Genomics 35, 425-40

A. Chromatin Dynamics in Gene Regulation

B. Epigenetic Regulation in Bone Disease

C. Cancer Epigenomics

A. Environmental Toxicology: Toxicity assessment and underlying mechanism
B. Incidence of metabolic disease by chemical exposure
C. Human health risk assessment

Tran CM, Lee H, Lee B, Ra JS, Kim KT. Effects of the chorion on the developmental toxicity of organophosphate esters in zebrafish embryos. J Hazard Mater. 2021 Jan 5;401:123389. doi: 10.1016/j.jhazmat.2020.123389. Epub 2020 Jul 9. PMID: 32763690
miR-137 and miR-141 regulate tail defects in zebrafish embryos caused by triphenyl phosphate (TPHP). Tran CM, Kim KT. Environ Pollut. 2020 Jul;262:114286. doi: 10.1016/j.envpol.2020.114286. Epub 2020 Mar 4. PMID: 32443217
Moon J, Lee B, Ra JS, Kim KT. Predicting PBT and CMR properties of substances of very high concern (SVHCs) using QSAR models, and application for K-REACH. Toxicol Rep. 2020 Aug 15;7:995-1000. doi: 10.1016/j.toxrep.2020.08.014. eCollection 2020. PMID: 32874922.
Park CM, Kim KT, Rhyu DY. Low-concentration exposure to organochlorine pesticides (OCPs) in L6 myotubes and RIN-m5F pancreatic beta cells induces disorders of glucose metabolism. Toxicol In Vitro. 2020 Jun;65:104767. doi: 10.1016/j.tiv.2020.104767. Epub 2020 Jan 7. PMID: 31923579
Rhyu D, Lee H, Tanguay RL, Kim KT. Tris(1,3-dichloro-2-propyl)phosphate (TDCIPP) disrupts zebrafish tail fin development. Ecotoxicol Environ Saf. 2019 Oct 30;182:109449. doi: 10.1016/j.ecoenv.2019.109449. Epub 2019 Jul 27. PMID: 31398778
Lee S, Lee H, Kim KT. Lee S. Optimization of experimental conditions and measurement of oxygen consumption rate (OCR) in zebrafish embryos exposed to organophosphate flame retardants (OPFRs). Ecotoxicol Environ Saf. 2019 Oct 30;182:109377. doi: 10.1016/j.ecoenv.2019.109377. Epub 2019 Jun 26. PMID: 31254858
Gao Y, Lee H, Kwon OK, Tan M, Kim KT, Lee S. Global Proteomic Analysis of Lysine Succinylation in Zebrafish (Danio rerio). J Proteome Res. 2019 Oct 4;18(10):3762-3769. doi: 10.1021/acs.jproteome.9b00462. Epub 2019 Sep 16. PMID: 31483678
Gao Y, Lee H, Kwon OK, Cheng Z, Tan M, Kim KT, Lee S. Profiling of Histidine Phosphoproteome in Danio rerio by TiO2 Enrichment. Proteomics. 2019 May;19(9):e1800471. doi: 10.1002/pmic.201800471. Epub 2019 Apr 18. PMID: 30864180
Lee H, Lee J, Choi K, Kim KT. Lee H. Comparative analysis of endocrine disrupting effects of major phthalates in employed two cell lines (MVLN and H295R) and embryonic zebrafish assay. Environ Res. 2019 May;172:319-325. doi: 10.1016/j.envres.2019.02.033. Epub 2019 Feb 22. PMID: 30825681

1. Functional mitochondria in health and disease

2. Cellular differentiation and development

3. Drug mechanism-of action discovery

A. 2021 년
1. Choi Y, Seo H, Cho M, Kim J, Chung HS, Lee I, Kim MJ. Rutin inhibits DRP1-mediated mitochondrial fission and prevents ethanol-induced hepatotoxicity in HepG2 cells and zebrafish. Anim Cells Syst (Seoul). 2021 Feb 11;25(1):74-81. doi: 10.1080/19768354.2021.1882565.
2. Ka H; Seo H; Choi Y; Kim J; Cho J; Choi S; Park S; Han S; An J; Chung H; Yang Y; Kim MJ. Loss of splicing factor IK impairs normal skeletal muscle development. BMC biology (Accepted)

B. 2020년
1. Kim JH, Ko ES, Kim D, Park SH, Kim EJ, Rho J, Seo H, Kim MJ, Yang WM, Ha IJ, Park MJ, Lee JY. Cancer cell-specific anticancer effects of Coptis chinensis on gefitinib-resistant lung cancer cells are mediated through the suppression of Mcl-1 and Bcl-2. Int J Oncol. 2020 Jun;56(6):1540-1550. doi: 10.3892/ijo.2020.5025. Epub 2020 Mar 24.
2. Lee EJ, Ko JY, Oh S, Jun J, Mun H, Lim CJ, Seo S, Ko HW, Kim H, Oh YK, Ahn C, Kang M, Kim MJ, Yoo KH, Oh GT, Park JH. Autophagy induction promotes renal cyst growth in polycystic kidney disease. EBioMedicine. 2020 Oct;60:102986. doi: 10.1016/j.ebiom.2020.102986. Epub 2020 Sep 16.

C. 2019년
1. An J, Kim SH, Hwang D, Lee KE, Kim MJ, Yang EG, Kim SY, Chung HS. Caspase-4 disaggregates lipopolysaccharide micelles via LPS-CARD interaction. Sci Rep. 2019 Jan 29;9(1):826. doi: 10.1038/s41598-018-36811-4.
2. An BH, Jeong H, Kim JH, Park S, Jeong JH, Kim MJ, Chang M. Estrogen Receptor-Mediated Transcriptional Activities of Spent Coffee Grounds and Spent Coffee Grounds Compost, and Their Phenolic Acid Constituents. J Agric Food Chem. 2019 Aug 7;67(31):8649-8659. doi: 10.1021/acs.jafc.9b02452. Epub 2019 Jul 25.PMID: 31283213
3. Lim J, Nam S, Jeong JH, Kim MJ, Yang Y, Lee MS, Lee HG, Ryu JH, Lim JS. Kazinol U inhibits melanogenesis through the inhibition of tyrosinase-related proteins via AMP kinase activation. Br J Pharmacol. 2019 Mar;176(5):737-750. doi: 10.1111/bph.14560. Epub 2019 Jan 28. PMID: 30579288

D. 2018년
1. Kim MJ. Betaine enhances the cellular survival via mitochondrial fusion and fission factors, MFN2 and DRP1. Anim Cells Syst (Seoul). 2018 Aug 30;22(5):289-298. doi: 10.1080/19768354.2018.1512523. eCollection 2018. PMID: 30460110
2. Lee H, Li H, Kweon M, Choi Y, Kim MJ, Ryu JH. Isobavachalcone from Angelica keiskei Inhibits Adipogenesis and Prevents Lipid Accumulation. Int J Mol Sci. 2018 Jun 6;19(6):1693. doi: 10.3390/ijms19061693. PMID: 29882838
3. Jeong H, Lee J, Kim S, Yeo YY, So H, Wu H, Song YS, Jang CY, Kim HD, Kim MJ, Chang M. Hepatic Metabolism of Sakuranetin and Its Modulating Effects on Cytochrome P450s and UDP-Glucuronosyltransferases. Molecules. 2018 Jun 26;23(7):1542. doi: 10.3390/molecules23071542. PMID: 29949932
4. Park SH, Kim JH, Ko E, Kim JY, Park MJ, Kim MJ, Seo H, Li S, Lee JY. Resistance to gefitinib and cross-resistance to irreversible EGFR-TKIs mediated by disruption of the Keap1-Nrf2 pathway in human lung cancer cells. FASEB J. 2018 May 29:fj201800011R. doi: 10.1096/fj.201800011R. PMID: 29812969

Screening for efficacy and toxicity of cosmetic ingredients

Cosmetic formulation and stability evaluation

A. 2021
1. Jang YA, Kim BA. Nguyen UT , Nguyen LT , Kim BA , Choi MJ , Yang IJ , Shin HM. Protective Effect of Spirulina-Derived C-Phycocyanin against Ultraviolet B-Induced Damage in HaCaT Cells. Medicina 2021, 57(3), 273; https://doi.org/10.3390/medicina57030273

B. 2020 년
1. Nguyen UT , Nguyen LT , Kim BA , Choi MJ , Yang IJ , Shin HM. Natural Compound Mixture, Containing Emodin, Genipin, Chlorogenic Acid, Cimigenoside, and Ginsenoside Rb1, Ameliorates Psoriasis-Like Skin Lesions by Suppressing Inflammation and Proliferation in Keratinocytes. Evid Based Complement Alternat Med. 2020 Oct 22;2020:9416962. doi: 10.1155/2020/9416962. PubMed PMID: 33149756
2. Kim YH, Yang IJ, Nguyen LY, Gum SI, Yu S, Lee GJ, Kim BA, Jung JC, Park YJ. Effect of Diquafosol on Hyperosmotic Stress-induced Tumor Necrosis Factor-α and Interleukin-6 Expression in Human Corneal Epithelial Cells. Korean J Ophthalmol . 2020 Feb;34(1):1-10. doi: 10.3341/kjo.2019.0046.. PubMed PMID: 32037744

A. Zebrafish EEG and EMG detection and analysis
B. EEG in drug screening

A. 2020 년
1. Lee Y, Lee KJ, Jang JW, Lee SI, Kim S. An EEG system to detect brain signals from multiple adult zebrafish. Biosens Bioelectron. 2020 Sep 15;164:112315. doi: 10.1016/j.bios.2020.112315. Epub 2020 May 23. PMID: 32553349
2. Lee Y, Seo HW, Lee KJ, Jang JW, Kim S. A Microfluidic System for Stable and Continuous EEG Monitoring from Multiple Larval Zebrafish. Sensors. 2020 Oct 19;20(20):5903. doi: 10.3390/s20205903. PMID: 33086704
3. Cho SJ, Kang YJ, Kim S. High-throughput zebrafish intramuscular recording array. Sensors and Actuators B. 2020 Feb 1;304;127332. doi.org/10.1016/j.snb.2019.127332
B. 2017년
1. Cho SJ, Byun D, Nam TS, Choi SY, Lee BG, Kim MK, Kim S. Zebrafish as an animal model in epilepsy studies with multichannel EEG recordings. Sci Rep. 2017 Jun 8;7(1):3099. doi: 10.1038/s41598-017-03482-6.PMID: 28596539
2. Cho SJ, Byun D, Nam TS, Choi SY, Lee BG, Kim MK, Kim S. A 3D-Printed Sensor for Monitoring Biosignals in Small Animals. J Healthc Eng. 2017;2017:9053764. doi: 10.1155/2017/9053764. Epub 2017 Oct 25.PMID: 29209491

C. 2015년
1. Cho SJ, Nam TS, Byun D, Choi SY, Kim MK, Kim S. Zebrafish needle EMG: a new tool for high-throughput drug screens. J Neurophysiol. 2015 Sep;114(3):2065-70. doi: 10.1152/jn.00538.2015. Epub 2015 Jul 15.

A. Natural products for drug discovery
1. HTS
2. Regeneration and inflammation
3. Particular matter-related disease mechanism

A. 2020 년
1. Kim YS, Kim J, Kim CS, Lee IS, Jo K, Jung DH, Lee YM, Kim JS. The Herbal Combination CPA4-1 Inhibits Changes in Retinal Capillaries and Reduction of Retinal Occludin in db/db Mice. Antioxidants (Basel). 2020 Jul 16;9(7):627. doi:10.3390/antiox9070627. PMID: 32708791; PMCID: PMC7402168.
2. Pyun BJ, Kim YS*, Lee IS, Jung DH, Kim JH, Kim JS*. Osteomeles schwerinae Extract and Its Major Compounds Inhibit Methylglyoxal-Induced Apoptosis in Human Retinal Pigment Epithelial Cells. Molecules. 2020 Jun 3;25(11):2605. doi: 10.3390/molecules25112605. PMID: 32503323; PMCID:PMC7321095.
B. 2019 년
1. Kim CS*, Kim J*, Kim YS*, Jo K, Lee YM, Jung DH, Lee IS, Kim JH, Kim JS. Improvement in Diabetic Retinopathy through Protection against Retinal Apoptosis in Spontaneously Diabetic Torii Rats Mediated by Ethanol Extract of Osteomeles schwerinae C.K. Schneid. Nutrients. 2019 Mar 4;11(3):546. doi:10.3390/nu11030546. PMID: 30836664; PMCID: PMC6470872.
2. Kang NR, Pyun BJ, Jung DH, Lee IS, Kim CS, Kim YS*, Kim JS*. Pueraria lobata Extract Protects Hydrogen Peroxide-Induced Human Retinal Pigment Epithelial Cells Death and Membrane Permeability. Evid Based Complement Alternat Med. 2019 Aug 27;2019:5710289. doi: 10.1155/2019/5710289. PMID: 31534464; PMCID:PMC6732599.
C. 2018년
1. Kim J, Hyun SW, Lee IS, Jo K, Kim YS, Kim JS, Kim CS. Aster koraiensis extract lowers postprandial glucose in normoglycemic and high-fat-diet-induced obese mice. Food Sci Biotechnol. 2018 Oct 23;28(2):563-568. doi:10.1007/s10068-018-0497-8. PMID: 30956869; PMCID: PMC6431321.
2. Liu C*, Kim YS*, Kim J, Pattison J, Kamaid A, Miller YI. Modeling hypercholesterolemia and vascular lipid accumulation in LDL receptor mutant zebrafish. J Lipid Res. 2018 Feb;59(2):391-399. doi: 10.1194/jlr.D081521. Epub 2017 Nov 29. PMID: 29187523; PMCID: PMC5794413.
D. 2017년
1. Pyun BJ, Kim YS, Lee IS, Kim JS. Homonoia riparia and its major component, myricitrin, inhibit high glucose-induced apoptosis of human retinal pericytes. Integr Med Res. 2017 Sep;6(3):300-309. doi:10.1016/j.imr.2017.07.004. Epub 2017 Jul 27. PMID: 28951844; PMCID: PMC5605388.
E. 2016년
1. Jung SH*, Kim YS*, Kim JS. High glucose-induced changes in hyaloid-retinal vessels during early ocular development of zebrafish: a short-term animal model of diabetic retinopathy. Br J Pharmacol. 2016 Jan;173(1):15-26. doi: 10.1111/bph.13279. Epub 2015 Nov 28. Br J Pharmacol. 2016. PMID: 28925940

I am highly interested in achieving healthy aging via investigating genetic and molecular mechanisms of vertebrate aging. The turquoise killifish (Nothobranchius furzeri) is a main model organism for this due to its shortest lifespan among vertebrate model organisms up to date, rapid progression of aging with conserved aging symptoms and easy of transgenesis.

A. Investigating interaction of genetic and molecular mechanism between circadian system and aging in the turquoise killifish

B. Identifying biological function of protein complexes during aging: Ribosome

C. Development of age-associated disease model

Lee S, Nam HG, Kim Y The core circadian component, BMAL1, is maintained in the pineal gland of aged killifish brain, iScience. 2020 Dec 9;24(1):101905. doi: 10.1016/j.isci.2020.101905. eCollection 2021 Jan 22. PMID: 33385110 PMCID: PMC7770606 DOI: 10.1016/j.isci.2020.101905
Do E, Kim Y Anatomical identification of neuroendocrine systems in the Nothobranchius furzeri, bioRxiv, 2020, doi: https://doi.org/10.1101/2020.10.15.342014
Metge F Kim Y, Boucas J, Dieterich C, Valenzano DR Circular RNAs in the ageing African turquoise killifish. bioRxiv, 2019, doi: https://doi.org/10.1101/699694

Mental disorders

A. Proteoatasis
1. Ubiquitin proteasome system, Autophagy
2. RASopathy
3. Transgenesis

A. 2020 년
1. Wnt-PLC-IP3-Connexin-Ca2+ axis maintains ependymal motile cilia in zebrafish spinal cord. Zhang J, Chandrasekaran G, Li W, Kim DY, Jeong IY, Lee SH, Liang T, Bae JY, Choi I, Kang H, Maeng JS, Kim MK, Lee T, Park SW, Kim MJ, Kim HS, Ro H, Bae YC, Park HC, Choi EY, Choi SY.Zhang J, et al. Among authors: ro h. Nat Commun. 2020 Apr 20;11(1):1860. doi: 10.1038/s41467-020-15248-2.
2. march5 Governs the Convergence and Extension Movement for Organization of the Telencephalon and Diencephalon in Zebrafish Embryos. Jung J, Choi I, Ro H, Huh TL, Choe J, Rhee M.Jung J, et al. Among authors: ro h. Mol Cells. 2020 Jan 31;43(1):76-85. doi: 10.14348/molcells.2019.0210.
B. 2019년
1. Accelerated degradation of cFLIPL and sensitization of the TRAIL DISC-mediated apoptotic cascade by pinoresinol, a lignan isolated from Rubia philippinensis. Lee SR, Quan KT, Byun HS, Park I, Kang K, Piao X, Ju E, Ro H, Na M, Hur GM.Lee SR, et al. Among authors: ro h. Sci Rep. 2019 Sep 18;9(1):13505. doi: 10.1038/s41598-019-49909-0.

• Wound healing and regeneration

• Disease models

• Screening of biomaterials

• Toxicity

2020 년
Dinusha C Rajapaksha, Shan L Edirisinghe, Chamilani Nikapitiya, SHS Dananjaya, Hyo-Jung Kwun, Cheol-Hee Kim, Chulhong Oh, Do-Hyung Kang, Mahanama De Zoysa. Spirulina maxima derived pectin nanoparticles enhance the immunomodulation, stress tolerance, and wound healing in zebrafish. Mar Drugs. 2020 Nov 7;18(11):556. doi: 10.3390/md18110556. PMID: 33171870.

2019 년
D C M Kulatunga, S H S Dananjaya, Chamilani Nikapitiya, G I Godahewa, Jongki Cho, Cheol-Hee Kim, Jehee Lee, Mahanama De Zoysa. Stress-immune responses and DNA protection function of thioredoxin domain containing 12 in zebrafish (Danio rerio) Fish Shellfish Immunol. 2019 Jan;84:1030-1040. doi: 10.1016/j.fsi.2018.10.052. Epub 2018 Oct 23. PMID: 3035974.

A. 환경 중 잠재적 유해물질의 유해성 평가
1. 환경 중 내분비계 교란 물질의 유해성 평가
2. 환경 중 입자성 물질에 대한 유해성 평가: (초)미세플라스틱, 나노물질
3. 생활환경화학물질의 복합노출에 대한 독성 평가
4. 분자적 수준에서의 독성 기전 규명
B. 신규 화학물질 (의약품 및 화장품)의 유효성 평가
C. 표준시험법 개발
1. ISO 시험법 개발: 제거된 제브라피쉬를 이용한 나노물질의 독성 평가
2. OECD 시험법의 개선 및 개발: 나노물질에 대한 기존 OECD 시험법의 적용성 검토 및 개선
3. 국립환경과학원 고시화 제안: OECD 시험법 (TG NO. 236)의 국내 시험법으로 제안
D. 예측모델 개발
1. 환경 또는 인체 유해성의 조기 검출을 위한 분자적 수준의 마커 개발

A. 2020 년
1. Kim YS, Kim JK, Hanh BTB, Kim SY, Kim HJ, Kim YJ, Jeon SM, Park CR, Oh GT, Park JW, Kim JM, Jang J, Jo EK. The Peroxisome Proliferator-Activated Receptor α- Agonist Gemfibrozil Promotes Defense Against Mycobacterium abscessus Infections. Cells. 2020 Mar 6;9(3):648. doi: 10.3390/cells9030648. PMID: 32155958
2. Hanh BTB, Park JW, Kim TH, Kim JS, Yang CS, Jang K, Cui J, Oh DC, Jang J. Rifamycin O, An Alternative Anti-Mycobacterium abscessus Agent. Molecules. 2020 Mar 31;25(7):1597. doi: 10.3390/molecules25071597. PMID: 32244387.
3. Kim YS, Kim JK, Hanh BTB, Kim SY, Kim HJ, Kim YJ, Jeon SM, Park CR, Oh GT, Park JW, Kim JM, Jang J, Jo EK. The Peroxisome Proliferator-Activated Receptor α- Agonist Gemfibrozil Promotes Defense Against Mycobacterium abscessus Infections. Cells. 2020 Mar 6;9(3):648. doi: 10.3390/cells9030648. PMID: 32155958.
4. Hanh BTB, Kim TH, Park JW, Lee DG, Kim JS, Du YE, Yang CS, Oh DC, Jang J. Etamycin as a Novel Mycobacterium abscessus Inhibitor. Int J Mol Sci. 2020 Sep 21;21(18):E6908. doi: 10.3390/ijms21186908. PMID: 32967077.
5. Kwon YS, Jung JW, Kim YJ, Park CB, Shon JC, Kim JH, Park JW, Kim SG, Seo JS. Proteomic analysis of whole-body responses in medaka (Oryzias latipes) exposed to benzalkonium chloride. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2020 Jul 21:1-11. doi: 10.1080/10934529.2020.1796117. Online ahead of print. PMID: 32693679.
6. Kim YJ, Lee SH, Jeon SM, Silwal P, Seo JY, Hanh BTB, Park JW, Whang J, Lee MJ, Heo JY, Kim SH, Kim JM, Song GY, Jang J, Jo EK. Sirtuin 3 is essential for host defense against Mycobacterium abscessus infection through regulation of mitochondrial homeostasis. Virulence. 2020 Dec;11(1):1225-1239. doi: 10.1080/21505594.2020.1809961.PMID: 32835604.
7. Jung JW, Kang JS, Choi J, Park JW. Chronic toxicity of endocrine disrupting chemicals used in plastic products in Korean resident species: Implications for aquatic ecological risk assessment. Ecotoxicol Environ Saf. 2020 Apr 1;192:110309. doi: 10.1016/j.ecoenv.2020.110309. Epub 2020 Feb 12. PMID: 32061985.
B. 2019년
1. Kim TH, Hanh BTB, Kim G, Lee DG, Park JW, Lee SE, Kim JS, Kim BS, Ryoo S, Jo EK, Jang J. Thiostrepton: A Novel Therapeutic Drug Candidate for Mycobacterium abscessus Infection. Molecules. 2019 Dec 10;24(24):4511. doi: 10.3390/molecules24244511. PMID: 31835481.
2. Johari SA, Rasmussen K, Gulumian M, Ghazi-Khansari M, Tetarazako N, Kashiwada S, Asghari S, Park JW, Yu IJ. Introducing a new standardized nanomaterial environmental toxicity screening testing procedure, ISO/TS 20787: aquatic toxicity assessment of manufactured nanomaterials in saltwater Lakes using Artemia sp. nauplii. Toxicol Mech Methods. 2019 Feb;29(2):95-109. doi: 10.1080/15376516.2018.1512695. Epub 2018 Sep 27. PMID: 30113242.
3. Choi JS, Hong SH, Park JW. Evaluation of microplastic toxicity in accordance with different sizes and exposure times in the marine copepod Tigriopus japonicus. Mar Environ Res. 2019 Nov 9:104838. doi: 10.1016/j.marenvres.2019.104838. Online ahead of print. PMID: 31733910.
4. Kang JS, Ahn TG, Park JW. Perfluorooctanoic acid (PFOA) and perfluooctane sulfonate (PFOS) induce different modes of action in reproduction to Japanese medaka (Oryzias latipes). J Hazard Mater. 2019 Apr 15;368:97-103. doi: 10.1016/j.jhazmat.2019.01.034. Epub 2019 Jan 14. PMID: 30665113.
C. 2018년
1. Choi JS, Jung YJ, Hong NH, Hong SH, Park JW. Toxicological effects of irregularly shaped and spherical microplastics in a marine teleost, the sheepshead minnow (Cyprinodon variegatus). Mar Pollut Bull. 2018 Apr;129(1):231-240. doi: 10.1016/j.marpolbul.2018.02.039. Epub 2018 Feb 27. PMID: 29680542.
2. Kang JS, Park JW. Insight on cytotoxic effects of silver nanoparticles: Alternative androgenic transactivation by adsorption with DHT. Sci Total Environ. 2018 Mar 15;618:712-717. doi: 10.1016/j.scitotenv.2017.08.059. Epub 2017 Nov 10. PMID: 29129328
D. 2017년
1. Kwak JI, Park JW, An YJ. Effects of silver nanowire length and exposure route on cytotoxicity to earthworms. Environ Sci Pollut Res Int. 2017 Jun;24(16):14516-14524. doi: 10.1007/s11356-017-9054-x. Epub 2017 Apr 27. PMID: 28452028.
E. 2016년
1. Kang JS, Choi JS, Park JW. Transcriptional changes in steroidogenesis by perfluoroalkyl acids (PFOA and PFOS) regulate the synthesis of sex hormones in H295R cells. Chemosphere. 2016 Jul;155:436-443. doi: 10.1016/j.chemosphere.2016.04.070. Epub 2016 Apr 30. PMID: 27139122.
2. Kang JS, Bong J, Choi JS, Henry TB, Park JW. Differentially transcriptional regulation on cell cycle pathway by silver nanoparticles from ionic silver in larval zebrafish (Danio rerio). Biochem Biophys Res Commun. 2016 Oct 28;479(4):753-758. doi: 10.1016/j.bbrc.2016.09.139. Epub 2016 Sep 28. PMID: 27693782.
F. 2015년
1. Choi S, Kim S, Bae YJ, Park JW, Jung J. Size-dependent toxicity of silver nanoparticles to Glyptotendipes tokunagai. Environ Health Toxicol. 2015 May 14;30:e2015003. doi: 10.5620/eht.e2015003. eCollection 2015. PMID: 26184045.
G. 2014년
1. Kim WK, Lee SK, Park JW, Choi K, Cargo J, Schlenk D, Jung J. Integration of multi-level biomarker responses to cadmium and benzo[k]fluoranthene in the pale chub (Zacco platypus). Ecotoxicol Environ Saf. 2014 Dec;110:121-8. doi: 10.1016/j.ecoenv.2014.08.025. Epub 2014 Sep 16. PMID: 25217733.
2. Park J, Kim S, Yoo J, Lee JS, Park JW, Jung J. Effect of salinity on acute copper and zinc toxicity to Tigriopus japonicus: the difference between metal ions and nanoparticles. Mar Pollut Bull. 2014 Aug 30;85(2):526-31. doi: 10.1016/j.marpolbul.2014.04.038. Epub 2014 May 16. PMID: 24837323.
3. Kang JS, Choi JS, Kim WK, Lee YJ, Park JW. Estrogenic potency of bisphenol S, polyethersulfone and their metabolites generated by the rat liver S9 fractions on a MVLN cell using a luciferase reporter gene assay. Reprod Biol Endocrinol. 2014 Nov 4;12:102. doi: 10.1186/1477-7827-12-102. PMID: 25371027.
4. Jang MH, Kim WK, Lee SK, Henry TB, Park JW. Uptake, tissue distribution, and depuration of total silver in common carp (Cyprinus carpio) after aqueous exposure to silver nanoparticles. Environ Sci Technol. 2014 Oct 7;48(19):11568-74. doi: 10.1021/es5022813. Epub 2014 Sep 15. PMID: 25171685.
5. Kim WK, Park JW, Lim ES, Lee SK, Kim J, Kim S, Lee SW, Choi K, Jung J. Tissue-specific antioxidant responses in pale chub (Zacco platypus) exposed to copper and benzo[a]pyrene. Bull Environ Contam Toxicol. 2014 May;92(5):540-5. doi: 10.1007/s00128-014-1213-x. Epub 2014 Jan 30.PMID: 24477393.
6. Park JW, Oh JH, Kim WK, Lee SK. Toxicity of citrate-coated silver nanoparticles differs according to method of suspension preparation. Bull Environ Contam Toxicol. 2014 Jul;93(1):53-9. doi: 10.1007/s00128-014-1296-4. Epub 2014 May 20. PMID: 24841540.
7. Seo J, Kim S, Choi S, Kwon D, Yoon TH, Kim WK, Park JW, Jung J. Effects of physiochemical properties of test media on nanoparticle toxicity to Daphnia magna Straus. Bull Environ Contam Toxicol. 2014 Sep;93(3):257-62. doi: 10.1007/s00128-014-1337-z. Epub 2014 Jul 26. PMID: 25063370.
H. 2013년
I. 2012년
1. Park JW, Heah TP, Gouffon JS, Henry TB, Sayler GS. Global gene expression in larval zebrafish (Danio rerio) exposed to selective serotonin reuptake inhibitors (fluoxetine and sertraline) reveals unique expression profiles and potential biomarkers of exposure. Environ Pollut. 2012 Aug;167:163-70. doi: 10.1016/j.envpol.2012.03.039. Epub 2012 May 9. PMID: 22575097.
J. 2011년
1. Park JW, Henry TB, Ard S, Menn FM, Compton RN, Sayler GS. The association between nC60 and 17α-ethinylestradiol (EE2) decreases EE2 bioavailability in zebrafish and alters nanoaggregate characteristics. Nanotoxicology. 2011 Sep;5(3):406-16. doi: 10.3109/17435390.2010.525329. Epub 2010 Oct 25. PMID: 20969539.
K. 2010년
1. Park JW, Henry TB, Menn FM, Compton RN, Sayler G. No bioavailability of 17α-ethinylestradiol when associated with nC60 aggregates during dietary exposure in adult male zebrafish (Danio rerio). Chemosphere. 2010 Nov;81(10):1227-32. doi: 10.1016/j.chemosphere.2010.09.036. PMID: 20937515.
L. 2009년
1. Park JW, Tompsett AR, Zhang X, Newsted JL, Jones PD, Au DW, Kong R, Wu RS, Giesy JP, Hecker M. Advanced fluorescence in situ hybridization to localize and quantify gene expression in Japanese medaka (Oryzias latipes) exposed to endocrine-disrupting compounds. Environ Toxicol Chem. 2009 Sep;28(9):1951-62. doi: 10.1897/08-574.1. PMID: 19469586.
M. 2008년
1. Park JW, Tompsett A, Zhang X, Newsted JL, Jones PD, Au D, Kong R, Wu RS, Giesy JP, Hecker M. Fluorescence in situ hybridization techniques (FISH) to detect changes in CYP19a gene expression of Japanese medaka (Oryzias latipes). Toxicol Appl Pharmacol. 2008 Oct 15;232(2):226-35. doi: 10.1016/j.taap.2008.06.012. Epub 2008 Jul 2. PMID: 18644401.
2. Zhang X, Hecker M, Park JW, Tompsett AR, Newsted J, Nakayama K, Jones PD, Au D, Kong R, Wu RS, Giesy JP. Real-time PCR array to study effects of chemicals on the Hypothalamic-Pituitary-Gonadal axis of the Japanese medaka. Aquat Toxicol. 2008 Jul 7;88(3):173-82. doi: 10.1016/j.aquatox.2008.04.009. Epub 2008 Apr 24. PMID: 18534694.
3. Zhang X, Hecker M, Park JW, Tompsett AR, Jones PD, Newsted J, Au DW, Kong R, Wu RS, Giesy JP. Time-dependent transcriptional profiles of genes of the hypothalamic-pituitary-gonadal axis in medaka (Oryzias latipes) exposed to fadrozole and 17beta-trenbolone. Environ Toxicol Chem. 2008 Dec;27(12):2504-11. doi: 10.1897/08-082.1. PMID: 18693774.
4. Zhang X, Hecker M, Tompsett AR, Park JW, Jones PD, Newsted J, Au D, Kong R, Wu RS, Giesy JP. Responses of the medaka HPG axis PCR array and reproduction to prochloraz and ketoconazole. Environ Sci Technol. 2008 Sep 1;42(17):6762-9. doi: 10.1021/es800591t. PMID: 1880056.
N. 2007년
1. Park JW, Bradford CM, Rinchard J, Liu F, Wages M, Waters A, Kendall RJ, Anderson TA, Theodorakis CW. Uptake, elimination, and relative distribution of perchlorate in various tissues of channel catfish. Environ Sci Technol. 2007 Nov 1;41(21):7581-6. doi: 10.1021/es071365n. PMID: 18044545.
O. 2006년
1. Bradford CM, Park JW, Rinchard J, Anderson TA, Liu F, Theodorakis CW. Uptake and elimination of perchlorate in eastern mosquitofish. Chemosphere. 2006 Jun;63(9):1591-7. doi: 10.1016/j.chemosphere.2005.08.073. Epub 2005 Nov 21. PMID: 16303168.
2. Park JW, Rinchard J, Liu F, Anderson TA, Kendall RJ, Theodorakis CW. The thyroid endocrine disruptor perchlorate affects reproduction, growth, and survival of mosquitofish. Ecotoxicol Environ Saf. 2006 Mar;63(3):343-52. doi: 10.1016/j.ecoenv.2005.04.002. PMID: 16507371.
3. Theodorakis C, Rinchard J, Anderson T, Liu F, Park JW, Costa F, McDaniel L, Kendall R, Waters A. Perchlorate in fish from a contaminated site in east-central Texas. Environ Pollut. 2006 Jan;139(1):59-69. doi: 10.1016/j.envpol.2005.04.030. Epub 2005 Jul 1. PMID: 15993996.
4. Theodorakis CW, Rinchard J, Carr JA, Park JW, McDaniel L, Liu F, Wages M. Thyroid endocrine disruption in stonerollers and cricket frogs from perchlorate-contaminated streams in east-central Texas. Ecotoxicology. 2006 Feb;15(1):31-50. doi: 10.1007/s10646-005-0040-6. Epub 2005 Dec 9. PMID: 16341611.
5. Park JW, Hecker M, Murphy MB, Jones PD, Solomon KR, Van Der Kraak G, Carr JA, Smith EE, du Preez L, Kendall RJ, Giesy JP. Development and optimization of a Q-RT PCR method to quantify CYP19 mRNA expression in testis of male adult Xenopus laevis: comparisons with aromatase enzyme activity. Comp Biochem Physiol B Biochem Mol Biol. 2006 May;144(1):18-28. doi: 10.1016/j.cbpb.2006.01.003. Epub 2006 Feb 21. PMID: 16492402.
P. 2005년
1. Hecker M, Kim WJ, Park JW, Murphy MB, Villeneuve D, Coady KK, Jones PD, Solomon KR, Van Der Kraak G, Carr JA, Smith EE, du Preez L, Kendall RJ, Giesy JP; Syngenta Crop Protection Inc. Plasma concentrations of estradiol and testosterone, gonadal aromatase activity and ultrastructure of the testis in Xenopus laevis exposed to estradiol or atrazine. Aquat Toxicol. 2005 May 15;72(4):383-96. doi: 10.1016/j.aquatox.2005.01.008. PMID: 15848257.
2. Hecker M, Park JW, Murphy MB, Jones PD, Solomon KR, Van Der Kraak G, Carr JA, Smith EE, du Preez L, Kendall RJ, Giesy JP. Effects of atrazine on CYP19 gene expression and aromatase activity in testes and on plasma sex steroid concentrations of male African clawed frogs (Xenopus laevis). Toxicol Sci. 2005 Aug;86(2):273-80. doi: 10.1093/toxsci/kfi203. Epub 2005 May 18. PMID: 15901915.

1.Oligodendrocyte development & disease
2. Motor neuron development & disease
3. Novel neuropeptid screening & functional study

A. 2020년
Kim HK, Lee DW, Kim E, Jeong I, Kim S, Kim BJ, Park HC. “Notch Signaling Controls Oligodendrocyte Regeneration in the Injured Telencephalon of Adult Zebrafish” (2020). Exp Neurobiol. 2020 Dec 31;29(6):417-424. doi: 10.5607/en20050.

Choi SY, Lee JH, Chung AY, Jo Y, Shin JH, Park HC, Kim H, Lopez-Gonzalez R, Ryu JR, Sun W. “Prevention of mitochondrial impairment by inhibition of protein phosphatase 1 activity in amyotrophic lateral sclerosis” (2020). Cell Death Dis. 2020 Oct 21;11(10):888. doi: 10.1038/s41419-020-03102-8.

Lee DW, Kim E, Jeong I, Kim HK, Kim S, Park HC. “Schwann cells selectively myelinate primary motor axons via neuregulin-ErbB signaling” (2020). Glia, doi: 10.1002/glia.23871.

Zhang J, Chandrasekaran G, Li W, Kim DY, Jeong IY, Lee SH, Liang T, Bae JY, Choi I, Kang H, Maeng JS, Kim MK, Lee T, Park SW, Kim MJ, Kim HS, Ro H, Bae YC, Park HC, Choi EY, Choi SY. “Wnt-PLC-IP 3-Connexin-Ca 2+ axis maintains ependymal motile cilia in zebrafish spinal cord” (2020). Nature Communications 11(1):1860. doi: 10.1038/s41467-020-15248-2.

Cho EH, Huh HJ, Jeong I, Lee NY, Koh WJ, Park HC, Ki CS. “A nonsense variant in NME5 causes human primary ciliary dyskinesia with radial spoke defects (2020). Clinical Genetics 98(1):64-68. doi: 10.1111/cge.13742.
(Co-corresponding author)

Han E, Ho Oh K, Park S, Chan Rah Y, Park HC, Koun S, Choi J. “Analysis of behavioral changes in zebrafish (Danio rerio) larvae caused by aminoglycoside-induced damage to the lateral line and muscles” (2020). Neurotoxicology 78:134-142. doi: 10.1016/j.neuro.2020.03.005.

Nam TS, Zhang J, Chandrasekaran G, Jeong IY, Li W, Lee SH, Kang KW, Maeng JS, Kang H, Shin HY, Park HC, Kim S, Choi SY, Kim MK. “A zebrafish model of nondystrophic myotonia with sodium channelopathy” (2020). Neuroscience Letters 714:134579. doi: 10.1016/j.neulet.2019.134579.

B. 2019년
Shahapal A, Cho EB, Yong HJ, Jeong I, Kwak H, Lee JK, Kim W, Kim B, Park HC, Lee WS, Kim H, Hwang JI, Seong JY. “FAM19A5 Expression During Embryogenesis and in the Adult Traumatic Brain of FAM19A5-LacZ Knock-in Mice” (2019). Frontiers in Neuroscience 13:917. doi: 10.3389/fnins.2019.00917.

Jeong I, Kim E, Seong JY, Park HC. “Overexpression of Spexin 1 in the Dorsal Habenula Reduces Anxiety in Zebrafish” (2019). Frontiers in Neural Circuits. 13:53. doi: 10.3389/fncir.2019.00053. eCollection 2019.

Park MH, Kim AK, Manandhar S, Oh SY, Jang GH, Kang L, Lee DW, Hyeon DY, Lee SH, Lee HE, Huh TL, Suh SH, Hwang D, Byun K, Park HC, Lee YM. “CCN1 interlinks integrin and hippo pathway to autoregulate tip cell activity” (2019). Elife. 8. pii: e46012. doi: 10.7554/eLife.46012.

Rhee J, Han E, Nam KJ, Lim KH, Chan Rah Y, Park S, Koun S, Park HC, Choi J. “Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5 μm (PM 2.5) exposure using zebrafish (Danio rerio) models” (2019). Int J Pediatr Otorhinolaryngol. 126, 109611. doi: 10.1016/j.ijporl.2019.109611.

Kim M, Jo Y, Hong JH, Kim S, Yoon S, Song KD, Kang S, Lee B, Kim GH, Park HC, Choi W. “Label-free neuroimaging in vivo using synchronous angular scanning microscopy with single-scattering accumulation algorithm” (2019). Nature Communications. 10(1):3152. doi: 10.1038/s41467-019-11040-z.

Kim S, Chung AY, Na JE, Lee SJ, Jeong SH, Kim E, Sun W, Rhyu IJ, Park HC. “Myelin degeneration induced by mutant superoxide dismutase 1 accumulation promotes amyotrophic lateral sclerosis” (2019). Glia. doi: 10.1002/glia.23669. [Epub ahead of print]

Kim E, Jeong I, Chung AY, Kim S, Kwon SH, Seong JY, Park HC. “Distribution and neuronal circuit of spexin 1/2 neurons in the zebrafish CNS” (2019). Scientific Reports 22;9(1):5025.

Rim CH, Koun S, Park HC, Lee S, Kim CY. “Radioprotective effects of mistletoe extract in zebrafish embryos in vivo” (2019). Int J Radiat Biol. 5:1-10.

Rah YC, Park S, Koun S, Park HC, Choi J. “In vivo assay of the ethanol-induced embryonic hair cell loss and the protective role of the retinoic and folic acid in zebrafish larvae (Danio rerio)” (2019). Alcohol 75:113-121.

Shim JS, Kim B, Park HC*, Ryu JJ*. “Temporal Control of WNT Activity Regulates Tooth Number in Fish” (2019). Journal of Dental Research. 98(3):339-346.(Co-coresponding author)

A. Macrophage Mediated Diseases
1. Macrophages in Homeostatic Immune Function
2. Macrophages in Intestinal Inflammation and Resolution
3. Targeting Tumor-Associated Macrophages
4. Macrophage-Pathogen Interactions in Infectious Diseases

A. 2020 년
1. Quan H, Kim J, Na YR, Kim JH, Kim BJ, Kim BJ, Hong JJ, Hwang ES, Seok SH. Human Cytomegalovirus-Induced Interleukin-10 Production Promotes the Proliferation of Mycobacterium massiliense in Macrophages. Front Immunol. 2020 Sep 10;11:518605. PMID: 33013921 (공동교신저자)

2. Na YR, Jung D, Song J, Park JW, Hong JJ, Seok SH. Pyruvate dehydrogenase kinase is a negative regulator of interleukin-10 production in macrophages. J Mol Cell Biol. 2020 Jul 1;12(7):543-555. PMID: 31900478 (교신저자)

3. Na YR, Kwon JW, Kim DY, Chung H, Song J, Jung D, Quan H, Kim D, Kim JS, Ju YW, Han W, Ryu HS, Lee YS, Hong JJ, Seok SH. Protein Kinase A Catalytic Subunit Is a Molecular Switch that Promotes the Pro-tumoral Function of Macrophages. Cell Rep. 2020 May 12;31(6):107643. PMID: 32402274 (교신저자)

4. Quan H, Oh GC, Seok SH, Lee HY. Fimasartan, an angiotensin II receptor antagonist, ameliorates an in vivo zebrafish model of heart failure. Korean J Intern Med. 2020 Mar 13. Online ahead of print. PMID: 32164398 (공동교신저자)

5. Song J, Han D, Lee H, Kim DJ, Cho JY, Park JH, Seok SH. A Comprehensive Proteomic and Phosphoproteomic Analysis of Retinal Pigment Epithelium Reveals Multiple Pathway Alterations in Response to the Inflammatory Stimuli. Int J Mol Sci. 2020 Apr 25;21(9):3037. PMID: 32344885 (공동교신저자)

6. Kim BH, Kim YJ, Kim MH, Na YR, Jung D, Seok SH, Kim J, Kim HJ. Identification of FES as a novel radiosensitizing target in human cancers. Clin Cancer Res. 2020 Jan 1;26(1):265-273. Epub 2019 Oct 1. PMID: 31573955

B. 2019 년

7. Na YR, Stakenborg M, Seok SH, Matteoli G. Macrophages in intestinal inflammation and resolution: a potential therapeutic target in IBD. Nat Rev Gastroenterol Hepatol. 2019 Sep;16(9):531-543. Epub 2019 Jul 16. PMID: 31312042 (공동교신저자)

8. Kim H, Chung H, Kim J, Choi DH, Shin Y, Kang YG, Kim BM, Seo SU, Chung S, Seok SH. Macrophages-Triggered Sequential Remodeling of Endothelium-Interstitial Matrix to Form Pre-Metastatic Niche in Microfluidic Tumor Microenvironment. Adv Sci (Weinh). 2019 Apr 10;6(11):1900195. PMID: 31179226 (공동교신저자)

9. Oh S, Chung H, Chang S, Lee SH, Seok SH, Lee H. Effect of Mechanical Stretch on the DNCB-induced Proinflammatory Cytokine Secretion in Human Keratinocytes. Sci Rep. 2019 Mar 26;9(1):5156. PMID: 30914685 (공동교신저자)

10. Park JY, Song MG, Kim WH, Kim KW, Lodhi NA, Choi JY, Kim YJ, Kim JY, Chung H, Oh C, Lee YS, Kang KW, Im HJ, Seok SH, Lee DS, Kim EE, Jeong JM. Versatile and Finely Tuned Albumin Nanoplatform based on Click Chemistry. Theranostics. 2019 May 18;9(12):3398-3409. PMID: 31281486

A. 항암효능물질 발굴 및 기전연구
1. Cell based anti-cancer drug screening
2. Study of MOA
3. Evaluation of drug effect on Patient-derived organoid Model
B. 암발생기전의 연구
1. PLK4 and centrosomal proteins depended Centrosome biogenesis
2. Regulation of cycle

A. 2020 년
1. Han HJ, Park C, Hwang J, N R T, Kim SO, Han J, Woo M, B S, Ryoo IJ, Lee KH, Cha-Molstad H, Kwon YT, Kim BY, Soung NK. CPPF, A Novel Microtubule Targeting Anticancer Agent, Inhibits the Growth of a Wide Variety of Cancers. Int J Mol Sci. 2020 Jul 7;21(13):4800.
2. Shin EY, Park JH, You ST, Lee CS, Won SY, Park JJ, Kim HB, Shim J, Soung NK, Lee OJ, Schwartz MA, Kim EG. Integrin-mediated adhesions in regulation of cellular senescence. Sci Adv. 2020 May 6;6(19):eaay3909.
3. Chirumarry S, Soung NK, Han J, Kim EY, Ryu EK, Lee YH, Shin SY, Gunasekaran P, Bang JK. Antibacterial AZT derivative regulates metastasis of breast cancer cells. Eur J Med Chem. 2020 May 1;193:112233.
4. Kim HM, He L, Lee S, Park C, Kim DH, Han HJ, Han J, Hwang J, Cha-Molstad H, Lee KH, Ko SK, Jang JH, Ryoo IJ, Blenis J, Lee HG, Ahn JS, Kwon YT, Soung NK, Kim BY. Inhibition of osteoclasts differentiation by CDC2-induced NFATc1 phosphorylation. Bone. 2020 Feb;131:115153.
B. 2019년
1. Yim MS, Soung NK, Han EH, Min JY, Han H, Son EJ, Kim HN, Kim B, Bang JK, Ryu EK. Vitamin E-Conjugated Phosphopeptide Inhibitor of the Polo-Box Domain of Polo-Like Kinase 1. Mol Pharm. 2019 Dec 2;16(12):4867-4877.
2. Kim DH, Ahn JS, Han HJ, Kim HM, Hwang J, Lee KH, Cha-Molstad H, Ryoo IJ, Jang JH, Ko SK, Yang JO, Lee HG, Lee S, Song EJ, Kim JY, Huh YH, Kwon YT, Soung NK, Kim BY. Cep131 overexpression promotes centrosome amplification and colon cancer progression by regulating Plk4 stability. Cell Death Dis. 2019 Jul 29;10(8):570.
3. Kim DH, Kim HM, Huong PTT, Han HJ, Hwang J, Cha-Molstad H, Lee KH, Ryoo IJ, Kim KE, Huh YH, Ahn JS, Kwon YT, Soung NK, Kim BY. Enhanced anticancer effects of a methylation inhibitor by inhibiting a novel DNMT1 target, CEP 131, in cervical cancer. BMB Rep. 2019 May;52(5):342-347.

A. We develop new tools that allow us to map the brain circuit with high throughput and precision
1. Neuro-imaging: we develop optical imaging technologies for high throughput brain imaging
2. Biomedical image analysis: we develop AI and signal processing algorithms for analyzing biomedical images with an emphasis on analyzing brain activity and structure

A. 2020 년
1. Yoon YG, Wang Z, Pak N, Park D, Dai P, Kang JS, Suk HJ, Symvoulidis P, Guner-Ataman B, Wang K, and Boyden ES. Sparse decomposition light-field microscopy for high speed imaging of neuronal activity. Optica. Oct 2020. doi.org/10.1364/OPTICA.392805.
2. Shemesh OA, Linghu C, Piatkevich KD, Goodwin D, Celiker OT, Gritton HJ, Romano MF, Gao R, Yu CJ, Tseng HA, Bensussen S, Narayan S, Yang CT, Freifeld L, Siciliano CA, Gupta I, Wang J, Pak N, Yoon YG, Ullmann JFP, Guner-Ataman B, Noamany H, Sheinkopf ZR, Park WM, Asano S, Keating AE, Trimmer JS, Reimer J, Tolias AS, Bear MF, Tye KM, Han X, Ahrens MB, Boyden ES. Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator. Neuron. 2020 Aug 5;107(3):470-486.e11. doi: 10.1016/j.neuron.2020.05.029. Epub 2020 Jun 26. PMID: 32592656; PMCID: PMC7415598.
B. 2014-2019년
1. Piatkevich KD, Jung EE, Straub C, Linghu C, Park D, Suk HJ, Hochbaum DR, Goodwin D, Pnevmatikakis E, Pak N, Kawashima T, Yang CT, Rhoades JL, Shemesh O, Asano S, Yoon YG, Freifeld L, Saulnier JL, Riegler C, Engert F, Hughes T, Drobizhev M, Szabo B, Ahrens MB, Flavell SW, Sabatini BL, Boyden ES. A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. Nat Chem Biol. 2018 Apr;14(4):352-360. doi: 10.1038/s41589-018-0004-9. Epub 2018 Feb 26. Erratum in: Nat Chem Biol. 2018 Mar 8;: PMID: 29483642; PMCID: PMC5866759.
2. Yoon YG, Dai P, Wohlwend J, Chang JB, Marblestone AH, Boyden ES. Feasibility of 3D Reconstruction of Neural Morphology Using Expansion Microscopy and Barcode-Guided Agglomeration. Front Comput Neurosci. 2017 Oct 24;11:97. doi: 10.3389/fncom.2017.00097. PMID: 29114215; PMCID: PMC5660712.
3. Chang JB, Chen F, Yoon YG, Jung EE, Babcock H, Kang JS, Asano S, Suk HJ, Pak N, Tillberg PW, Wassie AT, Cai D, Boyden ES. Iterative expansion microscopy. Nat Methods. 2017 Jun;14(6):593-599. doi: 10.1038/nmeth.4261. Epub 2017 Apr 17. PMID: 28417997; PMCID: PMC5560071.
4. Prevedel R, Yoon YG, Hoffmann M, Pak N, Wetzstein G, Kato S, Schrödel T, Raskar R, Zimmer M, Boyden ES, Vaziri A. Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy. Nat Methods. 2014 Jul;11(7):727-730. doi: 10.1038/nmeth.2964. Epub 2014 May 18. PMID: 24836920; PMCID: PMC4100252.

A. Hematopoiesis
Hematopoietic stem cells (HSCs) are multipotent progenitors of blood cells that self-renew and differentiate into all mature blood lineages. Therefore, strict control of the temporal and spatial specification, maturation, and expansion of HSCs is crucial for the normal function of the blood and immune system. HSCs arise from the hemogenic endothelium, a special population of endothelial cells within the ventral wall of the dorsal aorta that transdifferentiate into HSCs. Our studies are aimed at understanding how HSCs are specified. We performed forward genetic screen using zebrafish embryos to find novel genes required for HSC specification, obtaining multiple mutants, which failed to form HSCs in the aorta. We are currently characterizing the mutant phenotypes and are mapping and positionally cloning genes, which cause the defects of HSC formation. Our studies will provide insight into the molecular cues that specify HSCs.

B. DNA repair
Although diverse molecular and cellular biology studies have revealed numerous biological mechanisms involved in DNA damage responses and repair processes, understanding the roles of DNA repair genes at the level of the entire organisms remains limited. Zebrafish is a powerful genetic model system allowing for an array of diverse experimental approaches including large-scale forward screening and gene-editing through CRISPR targeting. Taking advantage of this model system, we generated multiple loss-of-function animals using high-throughput CRISPR mutagenesis. We obtained around 40 mutants of DNA repair genes and have continuously characterized the phenotypes of embryos and adult animals. Using these experimental tools, we will explore the in vivo roles and functions of DNA repair genes during development and eventually analyze the correlation of their functions with human genetic diseases.

Espanola SG, Song H, Ryu E, Saxena A, Kim E, Manegold JE, Nasamran CA, Sahoo D, Oh C, Bickers C, Shin U, Grainger S, Park YH, Pandolfo L, Kang M, Kang S, Myung K, Cooper KL, Yelon D, Traver D, Lee Y. 2020. Haematopoietic Stem Cell-Dependent Notch Transcription is Mediated by p53 through the Histone Chaperone Supt16h. Nat Cell Biol. In Press.

Shin U., Nakhro K, Oh C, Carrington B, Song H, Varshney G, Kim Y, Song H, Jeon S, Robbins G, Kim S, Yoon S, Choi Y, Park S, Kim YJ, Burgess S, Kang S, Sood R, Lee Y*, Myung K*. 2020. High-throughput phenotypic characterization of zebrafish CRISPR mutants of DNA repair genes. bioRxiv doi: https://doi.org/10.1101/2020.10.04.325621 (*Corresponding Authors).

A. Zebrafish AD modeling
1. Acute zebrafish AD model by Amyloid β delivery
2. Zebrafish tauopathy model by Tau overexpression
B. Host-microbe interaction studies
1. Microbiome dysbiosis regulated by a host mutation

A. 2019 년
1. Cho HJ, Lee JG, Kim JH, Kim SY, Huh YH, Kim HJ, Lee KS, Yu K, Lee JS. Vascular defects of DYRK1A knockouts are ameliorated by modulating calcium signaling in zebrafish. Dis Model Mech. 2019 May 23;12(5):dmm037044. doi: 10.1242/dmm.037044. PMID: 31043432; PMCID: PMC6550036
2. Lee WS , Cho HJ , Kim E , Huh YH , Kim HJ , Kim B , Kang T , Lee JS* , Jeong J*. Bioaccumulation of polystyrene nanoplastics and their effect on the toxicity of Au ions in zebrafish embryos. Nanoscale. 2019 Feb 14;11(7):3173-3185. doi: 10.1039/c8nr09321k. Erratum in: Nanoscale. 2019 Jan 4;: PMID: 30534785.
B. 2018 년
1. Gu GE, Park CS, Cho HJ, Ha TH, Bae J, Kwon OS, Lee JS*, Lee CS*. Fluorescent polydopamine nanoparticles as a probe for zebrafish sensory hair cells targeted in vivo imaging. Sci Rep. 2018 Mar 13;8(1):4393. doi: 10.1038/s41598-018-22828-2. PMID: 29535354; PMCID: PMC5849738..
C. 2017 년
1. Kim OH, Cho HJ, Han E, Hong TI, Ariyasiri K, Choi JH, Hwang KS, Jeong YM, Yang SY, Yu K, Park DS, Oh HW, Davis EE, Schwartz CE, Lee JS*, Kim HG*, Kim CH*. Zebrafish knockout of Down syndrome gene, DYRK1A, shows social impairments relevant to autism. Mol Autism. 2017 Sep 29;8:50. doi: 10.1186/s13229-017-0168-2. PMID: 29021890; PMCID: PMC5622473.
2. Shim J, Choi JH, Park MH, Kim H, Kim JH, Kim SY, Hong D, Kim S, Lee JE, Kim CH, Lee JS*, Bae YK*. Development of zebrafish medulloblastoma-like PNET model by TALEN-mediated somatic gene inactivation. Oncotarget. 2017 Jul 21;8(33):55280-55297. doi: 10.18632/oncotarget.19424. PMID: 28903419; PMCID: PMC5589658.
D. 2012 년
1. Zhu S*, Lee JS*, Guo F, Shin J, Perez-Atayde AR, Kutok JL, Rodig SJ, Neuberg DS, Helman D, Feng H, Stewart RA, Wang W, George RE, Kanki JP, Look AT. Activated ALK collaborates with MYCN in neuroblastoma pathogenesis. Cancer Cell. 2012 Mar 20;21(3):362-73. doi: 10.1016/j.ccr.2012.02.010. PMID: 22439933; PMCID: PMC3315700.
E. 2010 년
1. Lee JS*, Padmanabhan A*, Shin J, Zhu S, Guo F, Kanki JP, Epstein JA, Look AT. Oligodendrocyte progenitor cell numbers and migration are regulated by the zebrafish orthologs of the NF1 tumor suppressor gene. Hum Mol Genet. 2010 Dec 1;19(23):4643-53. doi: 10.1093/hmg/ddq395. Epub 2010 Sep 21. PMID: 20858602; PMCID: PMC3999377.
F. 2009 년
1. Padmanabhan A*, Lee JS*, Ismat FA, Lu MM, Lawson ND, Kanki JP, Look AT, Epstein JA. Cardiac and vascular functions of the zebrafish orthologues of the type I neurofibromatosis gene NFI. Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22305-10. doi: 10.1073/pnas.0901932106. Epub 2009 Dec 4. PMID: 19966217; PMCID: PMC2799742.
G. 2004 년
1. Lee JS, von der Hardt S, Rusch MA, Stringer SE, Stickney HL, Talbot WS, Geisler R, Nüsslein-Volhard C, Selleck SB, Chien CB, Roehl H. Axon sorting in the optic tract requires HSPG synthesis by ext2 (dackel) and extl3 (boxer). Neuron. 2004 Dec 16;44(6):947-60. doi: 10.1016/j.neuron.2004.11.029. PMID: 15603738.
2. Lee JS, Chien CB. When sugars guide axons: insights from heparan sulphate proteoglycan mutants. Nat Rev Genet. 2004 Dec;5(12):923-35. doi:10.1038/nrg1490. PMID: 15573124.
H. 2001 년
1. Lee JS, Ray R, Chien CB. Cloning and expression of three zebrafish roundabout homologs suggest roles in axon guidance and cell migration. Dev Dyn. 2001 Jun;221(2):216-30. doi: 10.1002/dvdy.1136. PMID: 11376489.

a. Disease modeling for Ciliopathies and neurological disorders
b. Development of drug screening system

1. So Yeon Won#, Soojin Kwon#, Hui Su Jeong, Ki Wha Chung, Byung-Ok Choi, Jong Wook Chang*, and Ji Eun Lee* (2020). Fibulin 5, a human WJ-MSCs-derived paracrine factor, attenuates PNS myelination defects through the Integrin-RAC1 signaling axis. Stem Cells (Epub) (Corresponding author)

2. Yong Joon Kim#, Eunji Jung#, Eunbie Shin, Sin-Hyoung Hong, Hui Su Jeong, Gayeong Hur, Hye Yun Jeong, Seung-Hyo Lee, Ji Eun Lee*, Gun-Hwa Kim*, and Joon Kim* (2020). Genome-wide RNA interference screening reveals a COPI-MAP2K3 pathway required for YAP regulation. Proc. Natl. Acad. Sci. USA 17 (33): 19994-20003 (Corresponding author)

3. Joon-Tae Kim#, So Yeon Won#, KyungWook Kang, Sang-Hoon Kim, Man-Seok Park, Kang-Ho Choi, Tai-Seung Nam, Simone W. Denis, Sacha Ferdinandusse, Ji Eun Lee*, Seok-Yong Choi*, Myeong-Kyu Kim* (2020). ACOX3 Dysfunction as a Potential Cause of Recurrent Spontaneous Vasospasm of Internal Carotid Artery. Translational Stroke Research 11 (5): 1041-1051 (Corresponding author)
4. Soo Mi Ki#, Ji Hyun Kim#, So Yeon Won#, Shin Ji Oh, In Young Lee, Young-Ki Bae, Ki Wha Chung, Byung-Ok Choi, Boyoun Park, Eui-Ju Choi, Ji Eun Lee (2020). CEP41-mediated ciliary tubulin glutamylation drives angiogenesis through AURKA-dependent deciliation. EMBO Reports 21(2):e48290. (Corresponding author)

A. Novel imaging techniques

1. Expansion microscopy

2. Super-resolution imaging

3. Multiplexed imaging

A. 2020 년
1. Park CE, Cho Y, Cho I, Jung H, Kim B, Shin JH, Choi S, Kwon SK, Hahn YK, Chang JB. Super-Resolution Three-Dimensional Imaging of Actin Filaments in Cultured Cells and the Brain via Expansion Microscopy. ACS Nano. 2020 Nov 24;14(11):14999-15010. doi: 10.1021/acsnano.0c04915. Epub 2020 Oct 23. PMID: 33095573

A. Biology and evolution of marine organisms
1. Molecular mechanisms that regulate development of marine organisms
2. Wound healing and regeneration
3. Genomics/Transcriptomics

B. Marine Biotechnology
1. Bioactive materials(peptide, natural compound) from marine organisms

A. 2020년
1. Jang DK, Pham CH, Lee IS, Jung SH, Jeong JH, Shin HS, Yoo HM. Anti-Melanogenesis Activity of 6-O-Isobutyrylbritannilactone from Inula britannica on B16F10 Melanocytes and In Vivo Zebrafish Models. Molecules. 2020 Aug 26;25(17):3887. doi: 10.3390/molecules25173887.
2. Jang DK, Jung SH, Jeong JH, Yoo HM, Lee IS, Shin HS. The Antimelanogenic Effect of Inularin Isolated from Flowers of Inula britannica on B16F10 Melanoma Cells and Zebrafish EmbryosJ Microbiol Biotechnol. 2020 May 28;30(5):749-752. doi: 0.4014/jmb.2003.03025.

B. 2019년
1. Song HY, Kim JK, Jo S, Jung SH, Kim B, Choi YJ, Yoo JS, Lee DS. The complete mitochondrial genome of Cynoglossus interruptus and its novel rearrangement (Pleuronectiformes: Cynoglossidae). Mitochondrial DNA B Resour. 2019 Sep 6;4(2):2924-2925. doi: 10.1080/23802359.2019.1660262.
2. Song HY, Choi YJ, Jo S, Kim B, Jung SH, Yoo JS, Lee DS. Characterization of the complete mitochondrial genome of Dactylopterus volitans (Syngnathiformes, Dactylopteridae). Mitochondrial DNA B Resour. 2019 Dec 23;5(1):351-352. doi: 10.1080/23802359.2019.1704651.
3. Song HY, Kim JK, Jo S, Jung SH, Lee DS, Kim B, Choi YJ, Yoo JS. Gene rearrangements in the mitochondrial genome of robust tonguefish, Cynoglossus robustus (Pleuronectiformes: Cynoglossidae) and a comparative analysis with other Cynoglossus fishes. Mitochondrial DNA B Resour. 2019 Dec 23;5(1):353-354. doi: 10.1080/23802359.2019.1637297.

C. 2018년
1. Choi JH, Jeong YM, Kim S, Lee B, Ariyasiri K, Kim HT, Jung SH, Hwang KS, Choi TI, Park CO, Huh WK, Carl M, Rosenfeld JA, Raskin S, Ma A, Gecz J, Kim HG, Kim JS, Shin HC, Park DS, Gerlai R, Jamieson BB, Kim JS, Iremonger KJ, Lee SH, Shin HS, Kim CH. Targeted knockout of a chemokine-like gene increases anxiety and fear responses. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):E1041-E1050. doi: 10.1073/pnas.1707663115
2. Lee IS, Jung SH, Kim JS. Polyphenols from Euphorbia pekinensis Inhibit AGEs Formation In Vitro and Vessel Dilation in Larval Zebrafish In Vivo. Planta Med. 2018 Feb;84(3):176-181. doi: 10.1055/s-0043-120447.
3. Jung SH, Song HY, Hyun YS, Kim YC, Whang I, Choi TY, Jo S. A Brain Atlas of the Long Arm Octopus, Octopus minor. Exp Neurobiol. 2018 Aug;27(4):257-266. doi: 10.5607/en.2018.27.4.257.
4. Kim BM, Kang S, Ahn DH, Jung SH, Rhee H, Yoo JS, Lee JE, Lee S, Han YH, Ryu KB, Cho SJ, Park H, An HS. The genome of common long-arm octopus Octopus minor. Gigascience. 2018 Nov 1;7(11):giy119. doi: 10.1093/gigascience/giy119.
5. Song HY, Jo S, Jung SH, Hwang HJ, An HS. Complete mitochondrial genome of Cheilio inermis (Labriformes, Labridae). Mitochondrial DNA B Resour. 2018 Aug 17;3(2):935-936. doi: 10.1080/23802359.2018.1501319.
6. Yun SJ, Kim H, Jung SH, Kim JH, Ryu JE, Singh NJ, Jeon J, Han JK, Kim CH, Kim S, Jang SK, Kim WJ. The mechanistic insight of a specific interaction between 15d-Prostaglandin-J2 and eIF4A suggests an evolutionary conserved role across species. Biol Open. 2018 Nov 13;7(11):bio035402. doi: 10.1242/bio.035402.

D. 2017년
1. Lee IS, Kim YJ, Jung SH, Kim JH, Kim JS. Flavonoids from Litsea japonica Inhibit AGEs Formation and Rat Lense Aldose Reductase In Vitro and Vessel Dilation in Zebrafish. Planta Med. 2017 Feb;83(3-04):318-325. doi: 10.1055/s-0042-116324..
2. Song HY, Hyun YS, Jo S, Jung SH, Hwang HJ, Kim SY, An HS. Complete mitochondrial genome of Parupeneus barberinus (Perciformes, Mullidae): mitogenome characterization and phylogenetic analysis. Mitochondrial DNA B Resour. 2017 Nov 7;2(2):785-786. doi: 10.1080/23802359.2017.1398613.

E. 2016년
1. Jung SH, Kim YS, Lee YR, Kim JS. High glucose-induced changes in hyaloid-retinal vessels during early ocular development of zebrafish: a short-term animal model of diabetic retinopathy. Br J Pharmacol. 2016 Jan;173(1):15-26. doi: 10.1111/bph.13279.

F. 2015년
1. Lee IS, Jung SH, Lee YM, Choi SJ, Sun H, Kim JS. Phenolic Compounds from the Leaves and Twigs of Osteomeles schwerinae That Inhibit Rat Lens Aldose Reductase and Vessel Dilation in Zebrafish Larvae. J Nat Prod. 2015 Sep 25;78(9):2249-54. doi: 10.1021/acs.jnatprod.5b00469.
2. Lee IS, Kim YS, Jung SH, Yu SY, Kim JH, Sun H, Kim JS. Lignans from the stems and leaves of Brandisia hancei and their effects on VEGF-induced vascular permeability and migration of HRECs and DLAV formation in zebrafish. Biosci Biotechnol Biochem. 2015;79(4):581-6. doi: 10.1080/09168451.2014.991687.

G. 2014년
1. So JH, Kim JD, Yoo KW, Kim HT, Jung SH, Choi JH, Lee MS, Jin SW, Kim CH. FIH-1, a novel interactor of mindbomb, functions as an essential anti-angiogenic factor during zebrafish vascular development. PLoS One. 2014 Oct 27;9(10):e109517. doi: 10.1371/journal.pone.0109517.
2. Kim YS, Jung SH, Jung DH, Choi SJ, Lee YR, Kim JS. Gas6 stimulates angiogenesis of human retinal endothelial cells and of zebrafish embryos via ERK1/2 signaling. PLoS One. 2014 Jan 7;9(1):e83901. doi: 10.1371/journal.pone.0083901.

H. 2013년
1. Yu SY, Lee IS, Jung SH, Lee YM, Lee YR, Kim JH, Sun H, Kim JS. Caffeoylated phenylpropanoid glycosides from Brandisia hancei inhibit advanced glycation end product formation and aldose reductase in vitro and vessel dilation in larval zebrafish in vivo. Planta Med. 2013 Dec;79(18):1705-9. doi: 10.1055/s-0033-1351101.
2. Lee IS, Yu SY, Jung SH, Lee YR, Lee YM, Kim JH, Sun H, Kim JS. Proanthocyanidins from Spenceria ramalana and their effects on AGE formation in vitro and hyaloid-retinal vessel dilation in larval zebrafish in vivo. J Nat Prod. 2013 Oct 25;76(10):1881-8. doi: 10.1021/np400442b.
3. Gadi J, Jung SH, Lee MJ, Jami A, Ruthala K, Kim KM, Cho NH, Jung HS, Kim CH, Lim SK. The transcription factor protein Sox11 enhances early osteoblast differentiation by facilitating proliferation and the survival of mesenchymal and osteoblast progenitors. J Biol Chem. 2013 Aug 30;288(35):25400-25413. doi: 10.1074/jbc.M112.413377.
4. Kim YI, Lee S, Jung SH, Kim HT, Choi JH, Lee MS, You KH, Yeo SY, Yoo KW, Kwak S, Lee JN, Park R, Choe SK, Kim CH. Establishment of a bone-specific col10a1:GFP transgenic zebrafish. Mol Cells. 2013 Aug;36(2):145-50. doi: 10.1007/s10059-013-0117-7.

I. 2012년
1. Jung SH, Kim HS, Ryu JH, Gwak JW, Bae YK, Kim CH, Yeo SY. Her4-positive population in the tectum opticum is proliferating neural precursors in the adult zebrafish brain. Mol Cells. 2012 Jun;33(6):627-32. doi: 10.1007/s10059-012-0091-5.
2. Kim KM, Park SJ, Jung SH, Kim EJ, Jogeswar G, Ajita J, Rhee Y, Kim CH, Lim SK. miR-182 is a negative regulator of osteoblast proliferation, differentiation, and skeletogenesis through targeting FoxO1. J Bone Miner Res. 2012 Aug;27(8):1669-79. doi: 10.1002/jbmr.1604.
3. Kim SN, Bae SJ, Kwak HB, Min YK, Jung SH, Kim CH, Kim SH. In vitro and in vivo osteogenic activity of licochalcone A. Amino Acids. 2012 Apr;42(4):1455-65. doi: 10.1007/s00726-011-0901-7.

J. 2011년
1. Kim HT, So JH, Jung SH, Ahn DG, Koh W, Kim NS, Kim SH, Lee S, Kim CH. Cug2 is essential for normal mitotic control and CNS development in zebrafish. BMC Dev Biol. 2011 Aug 15;11:49. doi: 10.1186/1471-213X-11-49.

K. 2010년
1. So JH, Hong SK, Kim HT, Jung SH, Lee MS, Choi JH, Bae YK, Kudoh T, Kim JH, Kim CH. Gicerin/Cd146 is involved in zebrafish cardiovascular development and tumor angiogenesis. Genes Cells. 2010 Nov;15(11):1099-110. doi: 10.1111/j.1365-2443.2010.01448.x.
2. Park SJ, Jung SH, Jogeswar G, Ryoo HM, Yook JI, Choi HS, Rhee Y, Kim CH, Lim SK. The transcription factor snail regulates osteogenic differentiation by repressing Runx2 expression. Bone. 2010 Jun;46(6):1498-507. doi: 10.1016/j.bone.2010.02.027.
3. Jung SH, Kim S, Chung AY, Kim HT, So JH, Ryu J, Park HC, Kim CH. Visualization of myelination in GFP-transgenic zebrafish. Dev Dyn. 2010 Feb;239(2):592-7. doi: 10.1002/dvdy.22166.

L. 2006년
1. Yoo KW, Kim EH, Jung SH, Rhee M, Koo BK, Yoon KJ, Kong YY, Kim CH. Snx5, as a Mind bomb-binding protein, is expressed in hematopoietic and endothelial precursor cells in zebrafish. FEBS Lett. 2006 Aug 7;580(18):4409-16. doi: 10.1016/j.febslet.2006.07.009.

A. Vascular Development and Endothelial Biology
B. Bone Morphogenetic Protein (BMP) signaling

Transcriptomic analysis identifies novel targets for individual bone morphogenetic protein type 1 receptors in endothelial cells. Choi W, Lee HW, Pak B, Han O, Kim M, Jin SW. FASEB J. 2021 Mar;35(3):e21386. doi: 10.1096/fj.202002071R. PMID: 33565137

Modulation of Endothelial Bone Morphogenetic Protein Receptor Type 2 Activity by Vascular Endothelial Growth Factor Receptor 3 in Pulmonary Arterial Hypertension. Hwangbo C, Lee HW, Kang H, Ju H, Wiley DS, Papangeli I, Han J, Kim JD, Dunworth WP, Hu X, Lee S, El-Hely O, Sofer A, Pak B, Peterson L, Comhair S, Hwang EM, Park JY, Thomas JL, Bautch VL, Erzurum SC, Chun HJ, Jin SW. Circulation. 2017 Jun 6;135(23):2288-2298. doi: 10.1161/CIRCULATIONAHA.116.025390. Epub 2017 Mar 29. PMID: 28356442

Notch regulates BMP responsiveness and lateral branching in vessel networks via SMAD6. Mouillesseaux KP, Wiley DS, Saunders LM, Wylie LA, Kushner EJ, Chong DC, Citrin KM, Barber AT, Park Y, Kim JD, Samsa LA, Kim J, Liu J, Jin SW, Bautch VL. Nat Commun. 2016 Nov 11;7:13247. doi: 10.1038/ncomms13247. PMID: 27834400

Bone morphogenetic protein 2 signaling negatively modulates lymphatic development in vertebrate embryos. Dunworth WP, Cardona-Costa J, Bozkulak EC, Kim JD, Meadows S, Fischer JC, Wang Y, Cleaver O, Qyang Y, Ober EA, Jin SW. Circ Res. 2014 Jan 3;114(1):56-66. doi: 10.1161/CIRCRESAHA.114.302452. Epub 2013 Oct 11. PMID: 24122719

Context-dependent proangiogenic function of bone morphogenetic protein signaling is mediated by disabled homolog 2. Kim JD, Kang H, Larrivée B, Lee MY, Mettlen M, Schmid SL, Roman BL, Qyang Y, Eichmann A, Jin SW. Dev Cell. 2012 Aug 14;23(2):441-8. doi: 10.1016/j.devcel.2012.07.007. PMID: 22898784

Distinct signalling pathways regulate sprouting angiogenesis from the dorsal aorta and the axial vein. Wiley DM, Kim JD, Hao J, Hong CC, Bautch VL, Jin SW. Nat Cell Biol. 2011 Jun;13(6):686-92. doi: 10.1038/ncb2232. Epub 2011 May 15. PMID: 21572418

Notch signaling functions as a cell-fate switch between the endothelial and hematopoietic lineages. Lee CY, Vogeli KM, Kim SH, Chong SW, Jiang YJ, Stainier DY, Jin SW. Curr Biol. 2009 Oct 13;19(19):1616-22. doi: 10.1016/j.cub.2009.07.073. Epub 2009 Sep 10. PMID: 19747827

A.Discovering unknown marine bioresources for pharmaceutical sciences

1. Search for candidate (hit) marine biomaterial to prevent and/or treat clinical diseases

A. 2020 년
1. Wang L, Lee W, Jayawardena TU, Cha SH, Jeon YJ. Dieckol, an algae-derived phenolic compound, suppresses airborne particulate matter-induced skin aging by inhibiting the expressions of pro-inflammatory cytokines and matrix metalloproteinases through regulating NF-κB, AP-1, and MAPKs signaling pathways. Food Chem Toxicol. 2020 Dec;146:111823. doi: 10.1016/j.fct.2020.111823. [Epub 2020 Oct 24] PubMed PMID: 33164846
2. Cha SH, Hwang Y, Heo SJ, Jun HS. Diphlorethohydroxycarmalol Attenuates Palmitate-Induced Hepatic Lipogenesis and Inflammation. Mar Drugs. 2020 Sep 18;18(9):475. doi: 10.3390/md18090475. PMID: 32962167
3. Phyu Khin P, Cha SH, Jun HS, Lee JH. A potential therapeutic combination for treatment of COVID-19: Synergistic effect of DPP4 and RAAS suppression. Med Hypotheses. 2020 Nov;144:110186. doi: 10.1016/j.mehy.2020.110186. Epub 2020 Aug 14. PMID: 33254506
4. Han EJ, Fernando IPS, Kim EA, Kim J, Jung K, Kim SY, Cha SH, Kim KN, Heo SJ, Ahn G. 5-Bromo-3,4-dihydroxybenzaldehyde from Polysiphonia morrowii attenuate IgE/BSA-stimulated mast cell activation and passive cutaneous anaphylaxis in mice. Biochem Pharmacol. 2020 Aug;178:114087. doi: 10.1016/j.bcp.2020.114087. Epub 2020 Jun 10. PMID: 32531348
5. Wang L, Kim HS, Je JG, Oh JY, Kim YS, Cha SH, Jeon YJ. Protective Effect of Diphlorethohydroxycarmalol Isolated from Ishige okamurae Against Particulate Matter-Induced Skin Damage by Regulation of NF-κB, AP-1, and MAPKs Signaling Pathways In Vitro in Human Dermal Fibroblasts. Molecules. 2020 Feb 26;25(5):1055. doi: 10.3390/molecules25051055. PMID: 32111060


B. 2019년
1. Hwang Y, Cha SH, Hong Y, Jung AR, Jun HS. Direct differentiation of insulin-producing cells from human urine-derived stem cells. Int J Med Sci. 2019 Nov 15;16(12):1668-1676. doi: 10.7150/ijms.36011. eCollection 2019. PMID: 31839754
2. Cha SH, Hwang Y, Heo SJ, Jun HS. Indole-4-carboxaldehyde Isolated from Seaweed, Sargassum thunbergii, Attenuates Methylglyoxal-Induced Hepatic Inflammation. Mar Drugs. 2019 Aug 21;17(9):486. doi: 10.3390/md17090486. PMID: 31438528
3. Kim D, Kim HJ, Cha SH, Jun HS. Protective Effects of Broussonetia kazinoki Siebold Fruit Extract against Palmitate-Induced Lipotoxicity in Mesangial Cells. Evid Based Complement Alternat Med. 2019 Jan 8;2019:4509403. doi: 10.1155/2019/4509403. eCollection 2019. PMID: 30728845
4. Cha SH, Han EJ, Ahn G, Jun HS. Taurine-Containing Hot Water Extract of Octopus Ocellatus Meat Prevents Methylglyoxal-Induced Vascular Damage. Adv Exp Med Biol. 2019;1155:471-482. doi: 10.1007/978-981-13-8023-5_44. PMID: 31468424
5. Lee W, Han EJ, Shin EJ, Han HJ, Ahn G, Cha SH. Protective Effect of Hot Water Extract of Loliolus Beka Gray Meat Against Palmitate-Induced HUVEC Damage. Adv Exp Med Biol. 2019;1155:717-727. doi: 10.1007/978-981-13-8023-5_62. PMID: 31468442
6. Lee W, Han EJ, Shin EJ, Han HJ, Ahn G, Cha SH. Hot Water Extract of Loliolus beka Meat Attenuates H2O2-Induced Damage in Human Umbilical Vein Endothelial Cells. Adv Exp Med Biol. 2019;1155:705-715. doi: 10.1007/978-981-13-8023-5_61. PMID: 31468441
7. Ko EY, Heo SJ, Cho SH, Lee W, Kim SY, Yang HW, Ahn G, Cha SH, Kwon SH, Jeong MS, Lee KP, Jeon YJ, Kim KN. 3‑Bromo‑5‑(ethoxymethyl)‑1,2‑benzenediol inhibits LPS-induced pro-inflammatory responses by preventing ROS production and downregulating NF-κB in vitro and in a zebrafish model. Int Immunopharmacol. 2019 Feb;67:98-105. doi:
10.1016/j.intimp.2018.11.021. [Epub 2018 Dec 8.] PMID: 30537636

A. Endocrine disruption
1. Thyroid disruption
2. Sex hormone disruption
B. Environmental toxicology
1. Consumer chemicals
2. Mixture toxicity
3. Ecotoxicology

A. 2019 년
1. Ji K, Seo J, Kho Y, Choi K. Co-exposure to ketoconazole alters effects of bisphenol A in Danio rerio and H295R cells. Chemosphere. 2019 Dec;237:124414. doi: 10.1016/j.chemosphere.2019.124414. Epub 2019 Jul 20.PMID: 31352099
2. Huda

A. Neurodevelopment
B. Drug screen

A. 2020 년
1. Zhang J, Chandrasekaran G, Li W, Kim DY, Jeong IY, Lee SH, Liang T, Bae JY, Choi I, Kang H, Maeng JS, Kim MK, Lee T, Park SW, Kim MJ, Kim HS, Ro H, Bae YC, Park HC, Choi EY, Choi SY. Wnt-PLC-IP3-Connexin-Ca2+ axis maintains ependymal motile cilia in zebrafish spinal cord. Nat Commun. 2020 Apr 20;11(1):1860. doi: 10.1038/s41467-020-15248-2.

B. 2019년
1. Jeong J, Kim KH, Kim DY, Chandrasekaran G, Kim M, Pagire SH, Dighe M, Choi EY, Bak SM, Kim EY, Shin MG, Choi SY, Ahn JH. Identification of new aryl hydrocarbon receptor (AhR) antagonists using a zebrafish model. Bioorg Med Chem. 2019 Oct 1;27(19):115014. doi: 10.1016/j.bmc.2019.07.030. Epub 2019 Jul 18.

A. Metabolic organelles
1. Peroxisome homeostasis and disease mechanism
2. Mitochondria metabolism and health
3. Organelle dysfunction leading to metabolic diseases

A. 2020 년
1. Maharjan Y, Lee JN, Kwak SA, Dutta RK, Park C, Choe SK, Park R. TMEM135 regulates primary ciliogenesis through modulation of intracellular cholesterol distribution. EMBO Rep. 2020 May 6;21(5):e48901. doi: 10.15252/embr.201948901. Epub 2020 Mar 11. PMID: 32157776; PMCID: PMC7202201.
2. Jo DS, Park SJ, Kim AK, Park NY, Kim JB, Bae JE, Park HJ, Shin JH, Chang JW, Kim PK, Jung YK, Koh JY, Choe SK, Lee KS, Cho DH. Loss of HSPA9 induces peroxisomal degradation by increasing pexophagy. Autophagy. 2020 Jan 22:1-15. doi: 10.1080/15548627.2020.1712812. Epub ahead of print. PMID: 31964216.
3. Kim YI, Nam IK, Lee DK, Bhandari S, Charton L, Kwak S, Lim JY, Hong K, Kim SJ, Lee JN, Kwon SW, So HS, Linka N, Park R, Choe SK. Slc25a17 acts as a peroxisomal coenzyme A transporter and regulates multiorgan development in zebrafish. J Cell Physiol. 2020 Jan;235(1):151-165. doi: 10.1002/jcp.28954. Epub 2019 Jun 11. PMID: 31187491.
B. 2019 년
1. Park WY, Choe SK, Park J, Um JY. Black Raspberry Promotes Browning of Preadipocytes and Inguinal White Adipose Tissue in Cold-Induced Mice. Nutrients. 2019 Sep 10;11(9):2164. doi: 10.3390/nu11092164. PMID: 31509935; PMCID: PMC6769844.
2. Kim HJ, Cao W, Oh GS, Lee S, Shen A, Khadka D, Lee SB, Sharma S, Kim SY, Choe SK, Kwak TH, Kim JM, Park R, So HS. Augmentation of cellular NAD⁺ by NQO1 enzymatic action improves age-related hearing impairment. Aging Cell. 2019 Oct;18(5):e13016. doi: 10.1111/acel.13016. Epub 2019 Jul 28. PMID: 31353811; PMCID: PMC6718544.
3. Choi YM, Kim YI, Choi JH, Bhandari S, Nam IK, Hong K, Kwak S, So HS, Park DS, Kim CH, Choi TY, Choe SK. Loss of abcd4 in zebrafish leads to vitamin B12-deficiency anemia. Biochem Biophys Res Commun. 2019 Jul 5;514(4):1264-1269. doi: 10.1016/j.bbrc.2019.05.099. Epub 2019 May 18. PMID: 31113616.
C. 2018 년
1. Shen A, Kim HJ, Oh GS, Lee SB, Lee S, Pandit A, Khadka D, Sharma S, Kim SY, Choe SK, Yang SH, Cho EY, Shim H, Park R, Kwak TH, So HS. Pharmacological stimulation of NQO1 decreases NADPH levels and ameliorates acute pancreatitis in mice. Cell Death Dis. 2018 Dec 18;10(1):5. doi: 10.1038/s41419-018-1252-z. PMID: 30584237; PMCID: PMC6315021.
2. Kim JK, Kim YS, Lee HM, Jin HS, Neupane C, Kim S, Lee SH, Min JJ, Sasai M, Jeong JH, Choe SK, Kim JM, Yamamoto M, Choy HE, Park JB, Jo EK. GABAergic signaling linked to autophagy enhances host protection against intracellular bacterial infections. Nat Commun. 2018 Oct 10;9(1):4184. doi: 10.1038/s41467-018-06487-5. PMID: 30305619; PMCID: PMC6180030.
3. Khadka D, Kim HJ, Oh GS, Shen A, Lee S, Lee SB, Sharma S, Kim SY, Pandit A, Choe SK, Kwak TH, Yang SH, Sim H, Eom GH, Park R, So HS. Augmentation of NAD+ levels by enzymatic action of NAD(P)H quinone oxidoreductase 1 attenuates adriamycin-induced cardiac dysfunction in mice. J Mol Cell Cardiol. 2018 Nov;124:45-57. doi: 10.1016/j.yjmcc.2018.10.001. Epub 2018 Oct 3. PMID: 30291911.
4. Park S, Oh J, Kim YI, Choe SK, Chun CH, Jin EJ. Suppression of ABCD2 dysregulates lipid metabolism via dysregulation of miR-141:ACSL4 in human osteoarthritis. Cell Biochem Funct. 2018 Oct;36(7):366-376. doi: 10.1002/cbf.3356. Epub 2018 Sep 27. PMID: 30264402.
5. Liu ZQ, Lee JN, Son M, Lim JY, Dutta RK, Maharjan Y, Kwak S, Oh GT, Byun K, Choe SK, Park R. Ciliogenesis is reciprocally regulated by PPARA and NR1H4/FXR through controlling autophagy in vitro and in vivo. Autophagy. 2018;14(6):1011-1027. doi: 10.1080/15548627.2018.1448326. Epub 2018 May 17. PMID: 29771182; PMCID: PMC6103415.
6. Lee JN, Dutta RK, Maharjan Y, Liu ZQ, Lim JY, Kim SJ, Cho DH, So HS, Choe SK, Park R. Catalase inhibition induces pexophagy through ROS accumulation. Biochem Biophys Res Commun. 2018 Jun 27;501(3):696-702. doi: 10.1016/j.bbrc.2018.05.050. PMID: 29753736.
7. Eun SY, Lee JN, Nam IK, Liu ZQ, So HS, Choe SK, Park R. PEX5 regulates autophagy via the mTORC1-TFEB axis during starvation. Exp Mol Med. 2018 Apr 6;50(4):4. doi: 10.1038/s12276-017-0007-8. PMID: 29622767; PMCID: PMC5938032.
8. Cho DH, Kim YS, Jo DS, Choe SK, Jo EK. Pexophagy: Molecular Mechanisms and Implications for Health and Diseases. Mol Cells. 2018 Jan 31;41(1):55-64. doi: 10.14348/molcells.2018.2245. Epub 2018 Jan 23. PMID: 29370694; PMCID: PMC5792714.

A. Hearing and balance
1. Ototoxicity screening and mechanism
2. Noise induced hearing loss
3. AI based hearing protective drug screening

A. 2020 년
1. Chang YS, Park SM, Rah YC, Han EJ, Koun SI, Chang J, Choi J. In vivo assessment of the toxicity of electronic cigarettes to zebrafish (Danio rerio) embryos, following gestational exposure, in terms of mortality, developmental toxicity, and hair cell damage: Toxicity of E-cigs to zebrafish embryos. Hum Exp Toxicol. 2020 Aug 10;:960327120947785. doi: 10.1177/0960327120947785. [Epub ahead of print] PubMed PMID: 32772711.
B. 2019년
1. Rhee J, Han E, Nam KJ, Lim KH, Chan Rah Y, Park S, Koun S, Park HC, Choi J. Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5 μm (PM 2.5) exposure using zebrafish (Danio rerio) models. Int J Pediatr Otorhinolaryngol. 2019 Nov;126:109611. doi: 10.1016/j.ijporl.2019.109611. Epub 2019 Jul 28. PubMed PMID: 313743

A. Biomaterials

B. Biopharmaceutics

C. Nanobiotechnology

A. Endodermal Organs(e.g., liver) development & regeneration, Hepatopancreaticobiliary & gastrointestinal diseases
1. The molecular mechanisms underlying biliary-driven liver regeneration
2. Generation of relevant animal models to understand hepatopancreaticobiliary and gastrointestinal diseases
3. Organ interaction when liver cells are damaged

A. 2020 년
1. KW Kim, TY Choi, YM Kwon, JYH Kim. Simple synthesis of photoluminescent carbon dots from a marine polysaccharide found in shark cartilage. E.J. Biotech.. 2020 sep doi: 10.1016/j.ejbt.2020.07.003.
B. 2019년
1. Kim, O.H., An, H.S., Choi, T.Y